RiboSubstrates: a web application addressing the cleavage specificities of ribozymes in designated genomes

Background: RNA-dependent gene silencing is becoming a routine tool used in laboratories worldwide. One of the important remaining hurdles in the selection of the target sequence, if not the most important one, is the designing of tools that have minimal off-target effects (i.e. cleaves only the desired sequence). Increasingly, in the current dawn of the post-genomic era, there is a heavy reliance on tools that are suitable for high-throughput functional genomics, consequently more and more bioinformatic software is becoming available. However, to date none have been designed to satisfy the ever-increasing need for the accurate selection of targets for a specific silencing reagent. Results: In order to overcome this hurdle we have developed RiboSubstrates http:// www.riboclub.org/ribosubstrates. This integrated bioinformatic software permits the searching of a cDNA database for all potential substrates for a given ribozyme. This includes the mRNAs that perfectly match the specific req...

• Type: Journal
• Year: 2006
• Where: BMCBI
• Authors: Jean-François Lucier, Lucien Junior Bergeron, Francis P. Brière, Rodney Ouellette, Sherif Abou Elela, Jean-Pierre Perreault
• Keywords: BMCBI 2006, Ribozymes, Routine Tool, Tool