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ICRA
2006
IEEE

High-speed Focusing of Cells using Depth-from-diffraction Method

14 years 5 months ago
High-speed Focusing of Cells using Depth-from-diffraction Method
— This paper proposes a new autofocusing method for microbiological specimens, such as cells. The proposed focusing method used a quick focus estimation named “depth from diffraction”, based on a diffraction pattern in a defocused image of a biological specimen. Since this method can estimate the focal position of the specimen from only a single defocused image, it can easily realize high-speed autofocusing. To demonstrate the proposed method, continuous focusing was applied to focus tracking of a swimming paramecium, in combination with twodimensional tracking. This allowed three-dimensional tracking of the paramecium for 75 s to be successfully demonstrated.
Hiromasa Oku, Koichi Hashimoto, Masatoshi Ishikawa
Added 11 Jun 2010
Updated 11 Jun 2010
Type Conference
Year 2006
Where ICRA
Authors Hiromasa Oku, Koichi Hashimoto, Masatoshi Ishikawa
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