TomoJ: tomography software for three-dimensional reconstruction in transmission electron microscopy

Background: Transmission electron tomography is an increasingly common three-dimensional electron microscopy approach that can provide new insights into the structure of subcellular components. Transmission electron tomography fills the gap between high resolution structural methods (X-ray diffraction or nuclear magnetic resonance) and optical microscopy. We developed new software for transmission electron tomography, TomoJ. TomoJ is a plug-in for the now standard image analysis and processing software for optical microscopy, ImageJ. Results: TomoJ provides a user-friendly interface for alignment, reconstruction, and combination of multiple tomographic volumes and includes the most recent algorithms for volume reconstructions used in three-dimensional electron microscopy (the algebraic reconstruction technique and simultaneous iterative reconstruction technique) as well as the commonly used approach of weighted back-projection. Conclusion: The software presented in this work is specif...

• Type: Journal
• Year: 2007
• Where: BMCBI
• Authors: Cédric Messaoudi, Thomas Boudier, Carlos Oscar Sánchez Sorzano, Sergio Marco
• Keywords: BMCBI 2007, Electron Microscopy Approach, Three-Dimensional Electron Microscopy, Transmission Electron Tomography