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BMCBI
2007

Metabonomics evaluations of age-related changes in the urinary compositions of male Sprague Dawley rats and effects of data norm

14 years 14 days ago
Metabonomics evaluations of age-related changes in the urinary compositions of male Sprague Dawley rats and effects of data norm
Background: Urine from male Sprague-Dawley rats 25, 40, and 80 days old was analyzed by NMR and UPLC/MS. The effects of data normalization procedures on principal component analysis (PCA) and quantitative analysis of NMR-based metabonomics data were investigated. Additionally, the effects of age on the metabolic profiles were examined by both NMR and UPLC/MS analyses. Results: The data normalization factor was shown to have a great impact on the statistical and quantitative results indicating the need to carefully consider how to best normalize the data within a particular study and when comparing different studies. PCA applied to the data obtained from both NMR and UPLC/MS platforms reveals similar age-related differences. NMR indicated many metabolites associated with the Krebs cycle decrease while citrate and 2-oxoglutarate, also associated with the Krebs cycle, increase in older rats. Conclusion: This study compared four different normalization methods for the NMR-based metabonomi...
Laura K. Schnackenberg, Jinchun Sun, Parvaneh Espa
Added 12 Dec 2010
Updated 12 Dec 2010
Type Journal
Year 2007
Where BMCBI
Authors Laura K. Schnackenberg, Jinchun Sun, Parvaneh Espandiari, Ricky D. Holland, Joseph Hanig, Richard D. Beger
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