Differential analysis for high density tiling microarray data

Background: High density oligonucleotide tiling arrays are an effective and powerful platform for conducting unbiased genome-wide studies. The ab initio probe selection method employed in tiling arrays is unbiased, and thus ensures consistent sampling across coding and non-coding regions of the genome. These arrays are being increasingly used to study the associated processes of transcription, transcription factor binding, chromatin structure and their association. Studies of differential expression and/or regulation provide critical insight into the mechanics of transcription and regulation that occurs during the developmental program of a cell. The time-course experiment, which comprises an in-vivo system and the proposed analyses, is used to determine if annotated and un-annotated portions of genome manifest coordinated differential response to the induced developmental program. Results: We have proposed a novel approach, based on a piece-wise function – to analyze genome-wide di...

• Type: Journal
• Year: 2007
• Where: BMCBI
• Authors: Srinka Ghosh, Heather A. Hirsch, Edward A. Sekinger, Philipp Kapranov, Kevin Struhl, Thomas R. Gingeras
• Keywords: BMCBI 2007, Developmental Program, Ensures Consistent Sampling, Unbiased Genome-wide Studies