Large-scale validation of methods for cytotoxic T-lymphocyte epitope prediction

Background: Reliable predictions of Cytotoxic T lymphocyte (CTL) epitopes are essential for rational vaccine design. Most importantly, they can minimize the experimental effort needed to identify epitopes. NetCTL is a web-based tool designed for predicting human CTL epitopes in any given protein. It does so by integrating predictions of proteasomal cleavage, TAP transport efficiency, and MHC class I affinity. At least four other methods have been developed recently that likewise attempt to predict CTL epitopes: EpiJen, MAPPP, MHC-pathway, and WAPP. In order to compare the performance of prediction methods, objective benchmarks and standardized performance measures are needed. Here, we develop such largescale benchmark and corresponding performance measures and report the performance of an updated

• Type: Journal
• Year: 2007
• Where: BMCBI
• Authors: Mette V. Larsen, Claus Lundegaard, Kasper Lamberth, Søren Buus, Ole Lund, Morten Nielsen
• Keywords: BMCBI 2007, Epitopes, Prediction, Rational Vaccine Design