Hybrid elementary flux analysis/nonparametric modeling: application for bioprocess control

Background: The progress in the "-omic" sciences has allowed a deeper knowledge on many biological systems with industrial interest. This knowledge is still rarely used for advanced bioprocess monitoring and control at the bioreactor level. In this work, a bioprocess control method is presented, which is designed on the basis of the metabolic network of the organism under consideration. The bioprocess dynamics are formulated using hybrid rigorous/data driven systems and its inherent structure is defined by the metabolism elementary modes. Results: The metabolic network of the system under study is decomposed into elementary modes (EMs), which are the simplest paths able to operate coherently in steady-state. A reduced reaction mechanism in the form of simplified reactions connecting substrates with end-products is obtained. A dynamical hybrid system integrating material balance equations, EMs reactions stoichiometry and kinetics was formulated. EMs kinetics were defined as t...

• Type: Journal
• Year: 2007
• Where: BMCBI
• Authors: Ana P. Teixeira, Carlos Alves, Paula M. Alves, Manuel J. T. Carrondo, Rui Oliveira
• Keywords: BMCBI 2007, Elementary Modes, EMs Kinetics, Metabolic Network