Splicy: a web-based tool for the prediction of possible alternative splicing events from Affymetrix probeset data

Background: The Affymetrix™ technology is nowadays a well-established method for the analysis of gene expression profiles in cancer research studies. However, changes in gene expression levels are not the only way to link genes and disease. The existence of gene isoforms specifically linked with cancer or apoptosis is increasingly found in literature. Hence it is of great interest to associate the results of a gene expression study with updated evidences on the transcript structure and its possible variants. Results: We present here a web-based software tool, Splicy, whose primary task is to retrieve data on the mapping of Affymetrix™ probes to single exons of gene transcripts and displaying graphically this information projected on the gene physical structure. Starting from a list of Affymetrix™ probesets the program produces a series of graphical displays, each relative to a transcript associated with the gene targeted by a given probe. The information on the transcript-by-tra...

• Type: Journal
• Year: 2007
• Where: BMCBI
• Authors: Davide Rambaldi, Barbara Felice, Viviane Praz, Philipp Bucher, Davide Cittaro, Alessandro Guffanti
• Keywords: BMCBI 2007, Gene, Gene Expression, Gene Expression Levels