ProMEX: a mass spectral reference database for proteins and protein phosphorylation sites

Background: In the last decade, techniques were established for the large scale genome-wide analysis of proteins, RNA, and metabolites, and database solutions have been developed to manage the generated data sets. The Golm Metabolome Database for metabolite data (GMD) represents one such effort to make these data broadly available and to interconnect the different molecular levels of a biological system [1]. As data interpretation in the light of already existing data becomes increasingly important, these initiatives are an essential part of current and future systems biology. Results: A mass spectral library consisting of experimentally derived tryptic peptide product ion spectra was generated based on liquid chromatography coupled to ion trap mass spectrometry (LC-IT-MS). Protein samples derived from Arabidopsis thaliana, Chlamydomonas reinhardii, Medicago truncatula, and Sinorhizobium meliloti were analysed. With currently 4,557 manually validated spectra associated with 4,226 uniq...

• Type: Journal
• Year: 2007
• Where: BMCBI
• Authors: Jan Hummel, Michaela Niemann, Stefanie Wienkoop, Waltraud X. Schulze, Dirk Steinhauser, Joachim Selbig, Dirk Walther, Wolfram Weckwerth
• Keywords: BMCBI 2007, Mass Spectral Library, Protein, Tryptic Peptides